clinical grade human adipose tissue-derived mesenchymal stem cell banking
نویسندگان
چکیده
in this study, our aim was to produce a generation of gmp-grade adipose tissue-derived mesenchymal stem cells for clinical applications. according to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cgmp) compliant adipose tissue-derived mesenchymal stem cells from five female donors. the isolated cells were cultured in dmem supplemented with 10% fetal bovine serum and characterized by standard methods. moreover, karyotyping was performed to evaluate chromosomal stability. mean of donors’ age was 47.6 ± 8.29 year, mean of cell viability was 95.6 ± 1.51%, and cell count was between 9×106 and 14×106 per microliter with the mean of 12.2×106 ± 2863564.21 per microliter. the main aim of this project was demonstrating the feasibility of cgmp-compliant and clinical grade adipose tissue-derived mesenchymal stem cells preparation and banking for clinical cell transplantation trials.
منابع مشابه
Clinical Grade Human Adipose Tissue-Derived Mesenchymal Stem Cell Banking.
In this study, our aim was to produce a generation of GMP-grade adipose tissue-derived mesenchymal stem cells for clinical applications. According to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cGMP) compliant adipose tissue-derived mesenchymal stem cells from five female donors. The isolated cells were cultured in DMEM supplemented...
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comparison of characteristics of human amniotic membrane and human adipose tissue derived mesenchymal stem cells
background mesenchymal stem cells (mscs) are ideal candidates for treatment of diseases. amniotic membranes are an inexpensive source of mscs (am-msc) without any donor site morbidity in cell therapy. adipose tissue derived stem cells (ascs) are also suitable cells for cell therapy. there is discrepancy in cd271 expression among mscs from different sources. in this study, the characteristics of...
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عنوان ژورنال:
acta medica iranicaجلد ۵۳، شماره ۹، صفحات ۵۴۰-۵۴۶
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